《植物生理学报》 2018, 54(5): 879-885

基于mRNA-Seq的沙漠植物花花柴干旱胁迫表达谱分析

王彦芹^1,2, 沈李丽^1,3, 罗来鑫³, 李健强³, 李志军^1,2,*

¹新疆生产建设兵团塔里木盆地生物资源保护利用重点实验室, 新疆阿拉尔843300; ²塔里木大学生命科学学院, 新疆阿拉尔843300; ³中国农业大学种子病理实验室, 北京100193

通信作者：李志军；E-mail: lizhijun0202@126.com

摘　要：

以抗逆性较强的荒漠植物花花柴为材料, 利用数字基因表达谱技术对用质量百分数20%的PEG溶液处理0、4、8、12和24 h后的花花柴幼苗的mRNA-Seq测序结果进行分析。结果表明, 花花柴幼苗叶部在不同处理时间均上调表达的基因有122个, 下调表达的基因54个, 根部在不同处理时间均上调表达的基因73个, 下调表达的基因79个。利用qRT-PCR和RT-PCR对可能与花花柴耐旱相关的6个差异表达基因进行了验证, 发现其表达模式与表达谱结果一致, 推测这些基因可能均与花花柴响应干旱胁迫相关。本研究为耐旱相关基因的发掘及应用提供了基础。

关键词：沙漠植物; 花花柴; 干旱胁迫; mRNA-Seq; 耐旱相关基因

收稿：2018-01-16   修定：2018-04-17

资助：新疆生产建设兵团科技计划项目(2012BB045)和国家自然科学基金(31460071和31660085)。

Analysis of expression profile in Karelinia caspia seedling under drought stress based on mRNA-Seq

WANG Yan-Qin^1,2, SHEN Li-Li^1,3, LUO Lai-Xin³, LI Jian-Qiang³, LI Zhi-Jun^1,2,*

¹The Xinjiang Production and Construction Corps Tarim Basin Key Laboratory of Biological Resources Protection, Alar, Xinjiang 843300, China; ²College of Life Sciences, Tarim University, Alar, Xinjiang 843300, China; ³The Seed Pathology Laboratory of China Agricultural University, Beijing 100193, China

Corresponding author: LI Zhi-Jun; E-mail: lizhijun0202@126.com

Abstract:

In this study, the gene expression profile of Karelinia caspica seedling was used for analysis under treatment with 20% PEG at 0, 4, 8, 12, and 24 h through mRNA-Seq technology. The results indicated that there were 122 genes up-regulated and 54 genes down-regulated expression in the leaves, and 73 genes up-regulated and 79 genes down-regulated expression in the roots of Karelinia caspica seedling under different time treatments. And then, 6 genes with differential expression were tested by qRT-PCR and RT-PCR. The result showed that the expression pattern of these 6 genes were as same as the mRNA-Seq. It was inferred that these genes might be related with drought stress in Karelinia caspica. This research provides a basis for the exploration and application of drought related genes from Karelinia caspica.

Key words: desert plant; Karelinia caspica; drought stress; mRNA-Seq; drought-related genes